Research
1) EPR Method Development
We develop methods in the area of Pulsed Dipolar EPR spectroscopy (PDS). For example, we aim at enabling EPR-based structure determination of biomolecules within cells and at room temperature. We also work on the methodology for determining distances to high-spin metal ions and for the localization of such ions in biomolecular structures. Last but not least and together with the group of U.B. Kaupp (LIMES, Bonn), we want to add the time domain to PDS-derived distance distributions and follow the process of conformational changes over time.
2) Synthesis
We synthesize new spin labels with tailored properties required for new EPR methods. This involves the synthesis of nitroxide and in particular trityl labels and their bioconjugation. In order to test the performance of new labels and EPR methods, we also synthesize organic, inorganic, and biomolecular model systems.
3) Systems
We are interested in unraveling conformational changes of biomolecules that are associated with their function, folding, and complex formation. We study this on a wide range of biomolecules ranging from metallo- and membraneproteins via riboswitches and ribozymes to RNA-protein complexes.
4) Service
We are always interested in applying our multifrequency cw/pulsed EPR methods to organic and inorganic molecules, solids or materials to help answer scientific questions. We are equipped with EPR in-situ electrochemical setups, flow systems for kinetic studies, and lamp/laser equipment for light excitation and time-resolved EPR.